瘢痕疙瘩间充质干细胞的分离与培养及其生长曲线的研究

目的探索获得瘢痕疙瘩来源的高纯度间充质干细胞(MSCs)的培养方法。方法来源于第四军医大学唐都医院皮肤科住院的4例患者的瘢痕疙瘩标本,所取病变部位均位于前胸及后背皮肤,采用DMEM∶F12/(100mL/L)胎牛血清(FBS)初步培养,再改用低糖DMEM/(100mL/L)FBS培养,接着用低糖DMEM/(10mL/L)FBS培养,最后用无血清的干细胞培养基(SCM)培养,并通过观察细胞形态特征及应用流式细胞术检测细胞表面标记物的表达情况进行鉴定。结果经血清梯度培养后得到了长梭形、形态均一,纯度较高的纤维样细胞,经流式细胞仪检测后细胞表面标记物表达情况为:CD29为99.99%,CD44为99.7%,CD45为0.69%,CD73为99.96%。结论血清浓度逐渐降低的梯度培养法可获得高纯度的瘢痕疙瘩MSCs。     

软弱土在预压荷载作用下的强度增长分析

从强度增长的公式出发,以实际工程为例,对影响软弱土强度增长的主要因素进行了探讨,并分析了预压荷载作用下软弱土实测强度增长值与规范计算值存在差异的主要原因。     

盐酸溶液中二环己基硫脲对304不锈钢的缓蚀作用

通过动电位极化曲线以及扫描电子显微镜观察,研究了四个温度下,二环己基硫脲（DCTU）对304L不锈钢在2 mol/L盐酸溶液中的缓蚀作用.结果表明：二环己基硫脲（DCTU）是304L不锈钢有效的阳极缓蚀剂,缓蚀效率随缓蚀剂的浓度和温度变化.二环己基硫脲（DCTU）在304L表面的吸附符合Temkeins吸附等温式,遵循化学吸附机理.相应的吸附动力学参数表明了金属基体与缓蚀剂间强烈的交互作用和自发吸附行为.     

加强供应链管理 构建节约型社会

以我国能源短缺为切入点,认为实施供应链管理可以使企业降低成本、创造竞争的整体优势,实现供求间的良好合作,为建设循环经济奠定基础。以实例说明了供应链管理给企业带来的显著经济效益。最后指出开展供应链管理对构建节约型社会、实现经济的可持续发展有重大意义。     

SS7C电力机车抱轴承箱结构的疲劳寿命分析

对SS7C电力机车抱轴承箱进行动应力实测 ,根据动应力实测编制了抱轴箱的二维应力谱 ,对抱轴箱的疲劳寿命进行了估算 ,同时也估算了抱轴箱体的裂纹扩展寿命和临界裂纹尺寸 ,为确定合理的检修周期提供依据。     

用现有疲劳试验数据确定疲劳裂纹扩展率

疲劳寿命的预报在船舶与海洋工程领域中相当重要，但其关键问题是要找到一种较科学的疲劳寿命预报方法。最近，本文第二作者提出了一种海洋结构物疲劳寿命预报的统一方法。该方法是基于疲劳裂纹扩展理论而发展起来的，在其九个参数模型的假设之下，能够较好地解释一些其它方法所不能解释的现象。采用该方法的主要障碍在于需要确定疲劳裂纹扩展率。作者通过对不同的疲劳裂纹扩展率的比较研究，并推广McEvily模型后，提出了一个具有较宽适用范围的九个参数疲劳裂纹扩展率模型(从门槛域一直到不稳定断裂域)。本文的主要目的是解决如何根据一些现有的疲劳试验数据来确定这九个模型参数的问题。文中给出了通过实验数据确定裂纹扩展率模型中各个参数的方法，并进行了模型参数的灵敏度分析。通过对文献中一些试验数据的收集，给出了几种常用金属材料的裂纹扩展率模型参数。     

论公路经营企业管理实践中财务管理的意义

分析公路经营企业中财务管理通过筹资决策、投资决策和股利决策来提高报酬率、降低风险、实现企业发展目标的策略。     

Logistical restructuring and road freight traffic growth

The forecasting of road freight traffic has relied heavily on the close correlation between GDP and road tonne-kilometers. It has not been rooted in an understanding of the causes of freight traffic growth. The research reported in this paper has investigated this process of traffic growth in two ways: first, by analysing official data on the production, consumption and movement of food and drink products, and second, by conducting a survey of the changing freight transport requirements of 88 large British-based manufacturers.The analysis of secondary data shows how, in the food and drink sector, the relationship between the real value of output and road vehicle-kms hinges on four key parameters: value density, handling factor, average length of haul and consignment size. An attempt is made to explain variations in these parameters.The survey of manufacturers suggests that the growth of lorry traffic is the net result of a complex interaction between factors operating at four levels of logistical management: strategic planning of logistical systems, choice of suppliers and distributors, scheduling of product flow and the management of transport resources. Changes in the frequency and scheduling of freight deliveries in response to tightening customer service requirements and just-in-time management appear to have become a more prevalent cause of freight traffic growth than the physical restructuring of logistical systems. Manufacturers anticipate that their road freight demand will broadly increase in line with sales and be largely unaffected by road transport cost increases at the levels currently proposed. The paper concludes by examining their likely reactions to a much sharper increase in the cost of road freight movement.     

断口定量分析840D车轮辐板孔裂纹扩展速率

对840D车轮辐板孔疲劳断口形貌进行观察并测量疲劳弧线间距。通过分析认为43 mm是临界裂纹的一个重要参考,同时发现闸瓦制动工况在裂纹的产生和扩展过程中起着重要的作用,并推断出裂纹长度在35 mm内,其平均裂纹扩展速率为31.2 mm/年。     

IMMUNOHISTOCHEMICAL LOCALIZATION OF EPIDERMAL GROWTH FACTOR RECEPTOR AND C－erbB－2 ONCOGENE PRODUCT IN DEVELOPING HUMAN PLACEN

ＩＭＭＵＮＯＨＩＳＴＯＣＨＥＭＩＣＡＬＬＯＣＡＬＩＺＡＴＩＯＮＯＦＥＰＩＤＥＲＭＡＬＧＲＯＷＴＨＦＡＣＴＯＲＲＥＣＥＰＴＯＲＡＮＤＣ－ｅｒｂＢ－２ＯＮＣＯＧＥＮＥＰＲＯＤＵＣＴＩＮＤＥＶＥＬＯＰＩＮＧＨＵＭＡＮＰＬＡＣＥＮＴＡ（马军）（王作清）（苏宝...